RT-PCR artifacts from processed pseudogenes.
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منابع مشابه
Sensitive and specific cytokeratin 18 reverse transcription-polymerase chain reaction that excludes amplification of processed pseudogenes from contaminating genomic DNA.
Processed pseudogenes of residual contaminating genomic DNA interfere with a sensitive detection of cytokeratin 18 (CK18) mRNA by reverse transcription and polymerase chain reaction (RT-PCR). This may cause false-positive results when CK18 mRNA is used as a marker for ectopic tumor cells in specimens from cancer patients. To establish a sensitive CK18 RT-PCR by excluding the amplification of pr...
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ورودعنوان ژورنال:
- PCR methods and applications
دوره 1 1 شماره
صفحات -
تاریخ انتشار 1991